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By the addition of plant or mammalian targeting sequences, green fluorescent protein (GFP) can be directed to the endoplasmic reticulum, the Golgi apparatus, or both organelles in plant cells. This unit describes the application of rapid Agrobacterium and virus-mediated transient expression systems in leaf tissue to permit visualization of ER and Golgi dynamics in vivo by epifluorescence or confocal microscopy.

Original publication

DOI

10.1002/0471143030.cb1303s09

Type

Journal article

Journal

Curr Protoc Cell Biol

Publication Date

05/2001

Volume

Chapter 13

Pages

Unit - 13.3

Keywords

Cell Migration Assays, Cell Movement, Endoplasmic Reticulum, Genetic Vectors, Golgi Apparatus, Green Fluorescent Proteins, Microscopy, Confocal, Microscopy, Fluorescence, Molecular Biology, Plant Proteins, Plant Viruses, Plants, Recombinant Fusion Proteins, Rhizobium, Staining and Labeling