Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We have previously developed a relatively simple, feeder-free method for deriving monocytes and macrophages from human Embryonic Stem cells, enabling the routine harvest of batches of up to 107 homogeneous monocytes from culture supernatants from a single standard 6-well tissue culture plate. Here we demonstrate further functional characteristics of these stem cell-derived macrophages. They are infectable with various strains of HIV-1, with appropriate replication kinetics, making them an attractive source of cells for studying HIV-macrophage interactions. They are also infectable with an important arbovirus, dengue virus. They suppress T cell proliferation, with tryptophan catabolism implicated in the process. We describe methods for genetically modifying the stem cells to maximise expression of genes-of-interest in the differentiated macrophages, addressing potential issues of transgene silencing transgene during differentiation. We also show that the method can be used to differentiate monocytes/ and macrophages from human induced Pluripotent Stem cells. This provides an accessible, reproducible, tractable system for investigating macrophage biology and host-pathogen interactions against known patient and control genetic backgrounds. © 2012 by Nova Science Publishers, Inc. All Rights Reserved.



Book title

Handbook of Macrophages: Life Cycle, Functions and Diseases

Publication Date



83 - 120