In vivo NMR analysis of tropane alkaloid metabolism in transformed root and de-differentiated cultures of Datura stramonium
Ford YY., Ratcliffe RG., Robins RJ.
In vivo 15 N NMR spectroscopy has been used to study the metabolism of [ 15 N]tropianone is transformed root cultures of Datura Stramonium. It was shown that, within only 2-4 hr of application of the labelled precursor, labelled metabolites can readily be detected. It was further demonstrate that de-differentiated cultures, derived from the roots by treatment with phytohirmones, are capable of metabolising [ 15 N]tropinone to [ 15 N]tropine and simple [ 15 N]tropine esters but that these cultures cannot convert [ 15 ]tropinone to [ 15 N]hyoscyamine.