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NMR investigations of nitrate in plant cells and tissues have hitherto been limited by the indistinguishability of the signals from intracellular and extracellular nitrate. Gd 3+ is shown to be an effective shift reagent for 14 N and 15 N nitrate NMR signals, resolving the internal and external nitrate signals in plant tissues, including cell suspensions and root material. However, time-course experiments show that, while the use of Gd 3+ allows nitrate levels to be monitored over extended periods, it also has adverse effects on growth and nitrate uptake. Accordingly, a number of chelated forms of gadolinium were investigated, and it is concluded that the NMR contrast agent Gd(DTPA-BMA) is likely to be a suitable shift reagent for physiologically relevant studies of nitrate transport in roots. © 1996 Academic Press, Inc.

Type

Journal article

Journal

Journal of Magnetic Resonance - Series B

Publication Date

01/01/1996

Volume

111

Pages

9 - 14