Optimization of steady-state 13C-labeling experiments for metabolic flux analysis
Kruger NJ., Masakapalli SK., Ratcliffe RG.
While steady-state 13 C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state 13 C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures. © Springer Science+Business Media New York 2014.