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While steady-state 13 C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state 13 C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures. © Springer Science+Business Media New York 2014.

Original publication

DOI

10.1007/978-1-62703-688-7-4

Type

Journal article

Journal

Methods in Molecular Biology

Publication Date

01/01/2014

Volume

1090

Pages

53 - 72