Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The uptake and compartmentation of manganese by maize roots, from solutions containing between 1 μM and 1 mM Mn2+, was monitored in vivo by31P nuclear magnetic resonance (NMR) spectroscopy. Qualitatively, NMR provided a convenient method for observing the effects of pH, anoxia, metabolic inhibitors, and competition with magnesium on the uptake of manganese and the resultshighlighted the role of the vacuole as a sink for Mn2+. Quantitatively, it was established thatroot tissues could maintain a low concentration of free Mn2+in the cytoplasm during manganese uptake and that there is a non-equilibrium distribution of Mn2+between the cytoplasm and the vacuole. Typically exposure to Mn2+in the range 10-100 μM resulted in a submicromolar pool of Mn2+in the cytoplasm and a vacuolar pool of 10 μM and it was concluded that the movement of Mn2+out of the cytoplasm must be energy consuming. Overall the results draw attention to the similarity between the subcellular distribution of manganese and calcium and provide some support for the suggestion that manganese, like calcium, might have a control function in normal cells. © 1993 Oxford University Press.

Original publication




Journal article


Journal of Experimental Botany

Publication Date





1819 - 1827