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The uptake and compartmentation of manganese by maize roots, from solutions containing between 1 μM and 1 mM Mn 2+ , was monitored in vivo by 31 P nuclear magnetic resonance (NMR) spectroscopy. Qualitatively, NMR provided a convenient method for observing the effects of pH, anoxia, metabolic inhibitors, and competition with magnesium on the uptake of manganese and the resultshighlighted the role of the vacuole as a sink for Mn 2+ . Quantitatively, it was established thatroot tissues could maintain a low concentration of free Mn 2+ in the cytoplasm during manganese uptake and that there is a non-equilibrium distribution of Mn 2+ between the cytoplasm and the vacuole. Typically exposure to Mn 2+ in the range 10-100 μM resulted in a submicromolar pool of Mn 2+ in the cytoplasm and a vacuolar pool of 10 μM and it was concluded that the movement of Mn 2+ out of the cytoplasm must be energy consuming. Overall the results draw attention to the similarity between the subcellular distribution of manganese and calcium and provide some support for the suggestion that manganese, like calcium, might have a control function in normal cells. © 1993 Oxford University Press.

Original publication

DOI

10.1093/jxb/44.12.1819

Type

Journal article

Journal

Journal of Experimental Botany

Publication Date

01/12/1993

Volume

44

Pages

1819 - 1827