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Non-coding transcripts play an important role in gene expression regulation in all species, including budding and fission yeast. Such regulatory transcripts include intergenic ncRNA (non-coding RNA), 5' and 3' UTRs, introns and antisense transcripts. In the present review, we discuss advantages and limitations of recently developed sequencing techniques, such as ESTs, DNA microarrays, RNA-Seq (RNA sequencing), DRS (direct RNA sequencing) and TIF-Seq (transcript isoform sequencing). We provide an overview of methods applied in yeast and how each of them has contributed to our knowledge of gene expression regulation and transcription.

Original publication

DOI

10.1042/BST20130144

Type

Journal article

Journal

Biochem Soc Trans

Publication Date

12/2013

Volume

41

Pages

1654 - 1659

Keywords

DNA, Fungal, Gene Expression Regulation, Fungal, RNA, Untranslated, Saccharomyces cerevisiae, Transcription, Genetic