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The electron paramagnetic resonance technique of double electron-electron resonance (DEER) was used to measure nanometre-scale distances between nitroxide spin labels attached to the complement regulatory protein CD55 (also known as decay accelerating factor) and the von Willebrand factor A (vWF-A) domain of factor B. Following a thorough assessment of the quality of the data, distances obtained from good-quality measurements are compared to predicted distances from a previously hypothesised model for the complex and are found to be incompatible. The success of using these distances as restraints in multi-body docking routines is presented critically.

Original publication

DOI

10.1080/00268976.2013.827754

Type

Journal article

Journal

Mol Phys

Publication Date

10/2013

Volume

111

Pages

2865 - 2872

Keywords

CD55, DEER, decay acceleration, distance restraints, vWF-A