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The Smchd1 gene encodes a large protein with homology to the SMC family of proteins involved in chromosome condensation and cohesion. Previous studies have found that Smchd1 has an important role in CpG island (CGI) methylation on the inactive X chromosome (Xi) and in stable silencing of some Xi genes. In this study, using genome-wide expression analysis, we showed that Smchd1 is required for the silencing of around 10% of the genes on Xi, apparently independent of CGI hypomethylation, and, moreover, that these genes nonrandomly occur in clusters. Additionally, we found that Smchd1 is required for CpG island methylation and silencing at a cluster of four imprinted genes in the Prader-Willi syndrome (PWS) locus on chromosome 7 and genes from the protocadherin-alpha and -beta clusters. All of the affected autosomal loci display developmentally regulated brain-specific methylation patterns which are lost in Smchd1 homozygous mutants. We discuss the implications of these findings for understanding the function of Smchd1 in epigenetic regulation of gene expression.

Original publication

DOI

10.1128/MCB.00145-13

Type

Journal article

Journal

Mol Cell Biol

Publication Date

08/2013

Volume

33

Pages

3150 - 3165

Keywords

Animals, Cadherins, Chromosomal Proteins, Non-Histone, CpG Islands, DNA Methylation, Embryo, Mammalian, Epigenesis, Genetic, Female, Gene Deletion, Gene Expression Regulation, Developmental, Genomic Imprinting, Male, Mice, Multigene Family, Prader-Willi Syndrome, Receptors, Cell Surface, X Chromosome