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Chemical probes have great potential for identifying functional residues in proteins in crude proteomes. Here we studied labeling sites of chemical probes based on sulfonyl fluorides (SFs) on plant and animal proteomes. Besides serine proteases and many other proteins, SF-based probes label Tyr residues in glutathione transferases (GSTs). The labeled GSTs represent four different GST classes that share less than 30% sequence identity. The targeted Tyr residues are located at similar positions in the promiscuous substrate binding site and are essential for GST function. The high selectivity of SF-based probes for functional Tyr residues in GSTs illustrates how these probes can be used for functional studies of GSTs and other proteins in crude proteomes.

Original publication

DOI

10.1016/j.chembiol.2013.01.016

Type

Journal article

Journal

Chem Biol

Publication Date

18/04/2013

Volume

20

Pages

541 - 548

Keywords

Animals, Binding Sites, Glutathione Transferase, Kinetics, Mice, Protein Structure, Tertiary, Proteome, Proteomics, Sulfinic Acids, Tyrosine