Regulatable vectors for environmental gene expression in Alphaproteobacteria.
Tett AJ., Rudder SJ., Bourdès A., Karunakaran R., Poole PS.
Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.