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Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

Original publication

DOI

10.1128/AEM.01188-12

Type

Journal article

Journal

Appl Environ Microbiol

Publication Date

10/2012

Volume

78

Pages

7137 - 7140

Keywords

Alphaproteobacteria, DNA, Bacterial, Environmental Microbiology, Gene Dosage, Gene Expression, Genetic Engineering, Genetic Vectors, Genetics, Microbial, Molecular Biology, Molecular Sequence Data, Plants, Plasmids, Promoter Regions, Genetic, Sequence Analysis, DNA