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Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

Original publication

DOI

10.1038/nmeth.2401

Type

Journal article

Journal

Nat Methods

Publication Date

04/2013

Volume

10

Pages

343 - 346

Keywords

Amino Acids, CD4-Positive T-Lymphocytes, Calcium Ionophores, Carcinogens, Chromatography, Liquid, Gene Expression Regulation, Humans, Ionomycin, Isotope Labeling, Phorbol Esters, Proteomics, Sensitivity and Specificity, Tandem Mass Spectrometry