Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Using primer-extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus. A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC-N4-ATTC. However, the major start site of transcription is a G at position -36 and this shorter transcript significantly increases in response to DNA damage by UV-light. The -35 box (TTGTCA) and -10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and this proximal promoter is almost identical to the strong, DNA damage-inducible promoter of Mycobacterium tuberculosis recA gene. We inspected the Streptomyces coelicolor database and found this type of promoter in the upstream regions of many (potentially) UV-inducible genes as well as some other genes/ORFs. Moreover, the DNA sequence between the predicted -35 and -10 boxes is also partially conserved. The consensus sequence for this new type of promoter in Streptomyces is: TTGTCAGTGGC-N6-TAGggT.

Type

Journal article

Journal

FEMS Microbiol Lett

Publication Date

19/03/2002

Volume

209

Pages

133 - 137

Keywords

Bacterial Proteins, Base Sequence, Consensus Sequence, DNA Damage, DNA Repair, DNA, Bacterial, Gene Expression Regulation, Bacterial, Genes, Bacterial, Open Reading Frames, Promoter Regions, Genetic, Rec A Recombinases, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity, Streptomyces, Transcription Initiation Site, Transcription, Genetic, Ultraviolet Rays