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Streptomyces RecA proteins are characterized by a conserved, positively charged extension of unknown function appended at their C-termini. To investigate the function of this element, we introduced the Streptomyces rimosus recA gene and its mutant form encoding the protein with a C-terminal deletion into S. rimosus. Both transcript and protein levels were dramatically increased in the strain expressing the truncated gene compared to the strain bearing the wild-type recA, indicating involvement of the characteristic C-terminal extension in regulating the recA expression in Streptomyces. Considering that RecA acts as a major regulator of DNA damage response in bacteria, this mode of regulation is expected to have broader implications and significance that outreaches our current understanding of RecA autoregulation.

Original publication

DOI

10.1016/j.femsle.2005.05.030

Type

Journal article

Journal

FEMS Microbiol Lett

Publication Date

01/07/2005

Volume

248

Pages

119 - 124

Keywords

Amino Acid Sequence, DNA Damage, Gene Deletion, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Phylogeny, Promoter Regions, Genetic, Rec A Recombinases, Streptomyces, Structure-Activity Relationship