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The metabolic effects of the administration of fructose to a yeast expressing the cDNA for rat liver ketohexokinase have been investigated by 31P-nuclear magnetic resonance spectroscopy. Cessation of growth suffered by the yeast on exposure to 5 and 25 mM-fructose was accompanied by a large accumulation of fructose 1-phosphate at the expense of cytoplasmic orthophosphate and nucleoside triphosphate. Shifts in resonances were consistent with a drop in cytoplasmic pH. Arresting growth with 1 mM-fructose, however, did not result in these changes, although a large accumulation of fructose 1-phosphate occurred which may have been supported by the mobilization of polyphosphates.

Original publication

DOI

10.1002/yea.320090807

Type

Journal article

Journal

Yeast

Publication Date

08/1993

Volume

9

Pages

867 - 873

Keywords

Animals, Fructokinases, Fructose, Fructose Intolerance, Magnetic Resonance Spectroscopy, Phosphorus Isotopes, Rats, Recombinant Proteins, Saccharomyces cerevisiae, Transformation, Genetic