Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The epidermal growth factor receptor (EGFR) has several functions in mammalian development and disease, particularly cancer. Most EGF ligands are synthesized as membrane-tethered precursors, and their proteolytic release activates signalling. In Drosophila, rhomboid intramembrane proteases catalyse the release of EGF-family ligands; however, in mammals this seems to be primarily achieved by ADAM-family metalloproteases. We report here that EGF is an efficient substrate of the mammalian rhomboid RHBDL2. RHBDL2 cleaves EGF just outside its transmembrane domain, thereby facilitating its secretion and triggering activation of the EGFR. We have identified endogenous RHBDL2 activity in several tumour cell lines.

Original publication

DOI

10.1038/embor.2011.50

Type

Journal article

Journal

EMBO Rep

Publication Date

05/2011

Volume

12

Pages

421 - 427

Keywords

Animals, Blotting, Western, COS Cells, Cell Line, Tumor, Cercopithecus aethiops, Epidermal Growth Factor, Green Fluorescent Proteins, Humans, Lentivirus, Mice, Microscopy, Fluorescence, Phenylalanine, Receptor, Epidermal Growth Factor, Serine Proteases, Signal Transduction, Substrate Specificity, Thiophenes, Transduction, Genetic