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Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. The results suggest that the hybridizing fragments contain single gene copies and permit the identification of the subunit encoded by each gene. Restriction-fragment variation associated with previously established allelic differences between varieties was observed. Also, there is a clear relationship between the electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it. These results are discussed with reference to the evolution of the HMW glutenin gene family and the uses of restriction-fragment variation in plant breeding and genetics.

Type

Journal article

Journal

Biochem Genet

Publication Date

08/1986

Volume

24

Pages

579 - 596

Keywords

Alleles, Amino Acid Sequence, Chromosome Mapping, DNA Restriction Enzymes, Genes, Genetic Variation, Glutens, Macromolecular Substances, Molecular Weight, Nucleic Acid Hybridization, Plants, Triticum