Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Active zones (AZs) are presynaptic membrane domains mediating synaptic vesicle fusion opposite postsynaptic densities (PSDs). At the Drosophila neuromuscular junction, the ELKS family member Bruchpilot (BRP) is essential for dense body formation and functional maturation of AZs. Using a proteomics approach, we identified Drosophila Syd-1 (DSyd-1) as a BRP binding partner. In vivo imaging shows that DSyd-1 arrives early at nascent AZs together with DLiprin-alpha, and both proteins localize to the AZ edge as the AZ matures. Mutants in dsyd-1 form smaller terminals with fewer release sites, and release less neurotransmitter. The remaining AZs are often large and misshapen, and ectopic, electron-dense accumulations of BRP form in boutons and axons. Furthermore, glutamate receptor content at PSDs increases because of excessive DGluRIIA accumulation. The AZ protein DSyd-1 is needed to properly localize DLiprin-alpha at AZs, and seems to control effective nucleation of newly forming AZs together with DLiprin-alpha. DSyd-1 also organizes trans-synaptic signaling to control maturation of PSD composition independently of DLiprin-alpha.

Original publication

DOI

10.1083/jcb.200908055

Type

Journal article

Journal

J Cell Biol

Publication Date

22/02/2010

Volume

188

Pages

565 - 579

Keywords

Animals, Drosophila Proteins, Drosophila melanogaster, Embryo, Nonmammalian, GTPase-Activating Proteins, Locomotion, Longevity, Mutation, Neuromuscular Junction, Phenotype, Phosphoproteins, Presynaptic Terminals, Protein Binding, Protein Transport, Proteomics, Receptors, Glutamate, Sequence Homology, Amino Acid, Synaptic Potentials