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To identify proteins that bind mammalian IAP homolog A (MIHA, also known as XIAP), we used coimmuno-precipitation and 2D immobilized pH gradient/SDS PAGE, followed by electrospray ionization tandem mass spectrometry. DIABLO (direct IAP binding protein with low pI) is a novel protein that can bind MIHA and can also interact with MIHB and MIHC and the baculoviral IAP, OpIAP. The N-terminally processed, IAP-interacting form of DIABLO is concentrated in membrane fractions in healthy cells but released into the MIHA-containing cytosolic fractions upon UV irradiation. As transfection of cells with DIABLO was able to counter the protection afforded by MIHA against UV irradiation, DIABLO may promote apoptosis by binding to IAPs and preventing them from inhibiting caspases.

Type

Journal article

Journal

Cell

Publication Date

07/07/2000

Volume

102

Pages

43 - 53

Keywords

Amino Acid Sequence, Animals, Apoptosis, Carrier Proteins, Cell Line, Transformed, Cloning, Molecular, Cysteine Proteinase Inhibitors, Gene Expression, Humans, Inhibitor of Apoptosis Proteins, Intracellular Signaling Peptides and Proteins, Mammals, Mice, Mitochondrial Proteins, Molecular Sequence Data, Nucleopolyhedrovirus, Proteins, Sequence Homology, Amino Acid, Subcellular Fractions, Viral Proteins, X-Linked Inhibitor of Apoptosis Protein