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Assembly of herpes simplex virus 1 (HSV-1) occurs in the cytoplasm, where the capsid and tegument bud into host cell membranes. It is at this point that the viral glycoproteins are incorporated into the virion, as they are located at the assembly site. We investigated the role of the Rab GTPases in coordinating the assembly process by overexpressing 37 human Rab GTPase-activating proteins (GAPs) and assessing infectious titers. Rab GTPases are key cellular regulators of membrane trafficking events that, by their membrane association and binding of effector proteins, ensure the appropriate fusion of membranes. We identified that TBC1D20 and RN-tre and their partner Rabs, Rab1a/b and Rab43, respectively, are important for virion assembly. In the absence of Rab1a/b, the viral glycoproteins are unable to traffic from the endoplasmic reticulum to the assembly compartment, and thus unenveloped particles build up in the cytoplasm. The defect resulting from Rab43 depletion is somewhat more complex, but it appears that the fragmentation and dispersal of the trans-Golgi network and associated membranes render these compartments unable to support secondary envelopment.

Original publication

DOI

10.1128/JVI.00500-11

Type

Journal article

Journal

J Virol

Publication Date

08/2011

Volume

85

Pages

8012 - 8021

Keywords

Adaptor Proteins, Signal Transducing, Animals, COS Cells, Cercopithecus aethiops, Cytoplasm, Endoplasmic Reticulum, Fluorescent Antibody Technique, GTPase-Activating Proteins, HeLa Cells, Herpesvirus 1, Human, Humans, Microscopy, Electron, RNA Interference, RNA, Small Interfering, Vero Cells, Viral Envelope Proteins, Virus Assembly, Virus Replication, rab GTP-Binding Proteins, rab1 GTP-Binding Proteins