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Primary cilia are sensory structures on the cell surface whose formation requires tight integration of microtubules and polarized membrane trafficking events. Rabs are GTP-binding proteins of the Ras superfamily that control directed membrane trafficking by regulating membrane-membrane and membrane-cytoskeleton interactions. This chapter describes cell biological and biochemical methods for the analysis of Rab function and Rab GTPase-activating proteins during primary cilia formation.

Original publication

DOI

10.1016/S0076-6879(07)00426-0

Type

Journal article

Journal

Methods Enzymol

Publication Date

2008

Volume

439

Pages

353 - 364

Keywords

Cell Line, Cilia, Cloning, Molecular, GTPase-Activating Proteins, Heat-Shock Proteins, Humans, Microtubule-Associated Proteins, Nuclear Proteins, rab GTP-Binding Proteins