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The RNA genome of influenza virus is transcribed and replicated by the viral RNA polymerase complex in the cell nucleus. We have generated green fluorescent protein (GFP)-tagged polymerase subunits to study the assembly of the polymerase complex. Our results show that individually expressed polymerase basic protein 1 (PB1) and polymerase acidic protein (PA) subunits were distributed in both the cytoplasm and the nucleus, while the polymerase basic protein 2 (PB2) subunit accumulated in the nucleus. Although it has been reported that PB1 alone accumulates in the nucleus, we demonstrate that PB1 requires the coexpression of PA for efficient nuclear accumulation. Our results support a model which proposes that PB1 and PA are transported into the nucleus as a complex.

Original publication




Journal article


J Virol

Publication Date





9144 - 9153


Active Transport, Cell Nucleus, Animals, Cell Line, Cell Nucleus, Cercopithecus aethiops, DNA-Directed RNA Polymerases, Humans, Influenza A virus, Models, Biological, Mutation, Nuclear Localization Signals, Protein Structure, Quaternary, Protein Subunits, RNA, Viral