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We have used a combination of centrifugation onto electron microscope grids and negative staining to study the structure of isolated mammalian centrioles. The technique relies on visualisation of structural detail by use of a goldthioglucose negative stain. The approach provides an easy structural definition of the mature and immature centriole and has revealed some novel proximal projections on the mature centriole. The rapid technique should prove of use in future analyses of centriolar structure and biochemistry.

Original publication

DOI

10.1006/jsbi.1996.0086

Type

Journal article

Journal

J Struct Biol

Publication Date

11/1996

Volume

117

Pages

222 - 226

Keywords

Animals, Aurothioglucose, Cell Fractionation, Centrioles, Microscopy, Electron, Sheep, Staining and Labeling, Thymus Gland