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T cell adhesion induced after physiological stimulation by antigen was investigated using murine T cell hybridomas specific for a tetanus toxin peptide. By employing a novel assay, the T cell hybridomas were shown to strongly adhere to peptide-pulsed APC in a dose-dependent fashion. Adhesion peaked at 30-60 min and declined thereafter. This assay allowed us to study the relationship between T cell adhesion and later activation responses using tetanus toxin peptide and alanine monosubstituted analogs. We show that the degree of peptide-induced T cell adhesion correlated with the magnitude of late functional responses. CD4, LFA-1 (CD11a/CD18), and CD28 were critical in the adhesion response. The enhancing role of CD4 was further demonstrated by reduced levels of T cell adhesion and late responses of CD4- T cell hybridomas. Reexpression of CD4 reversed these defects. Our data suggest a link between antigen-induced T cell adhesion and late responses and also suggest that signals mediated by TCR and CD4 coengagement may induce a greater activation and/or recruitment of molecules involved in T cell adhesion.

Original publication

DOI

10.1006/cimm.1996.0264

Type

Journal article

Journal

Cell Immunol

Publication Date

01/11/1996

Volume

173

Pages

165 - 175

Keywords

Animals, Antigen-Presenting Cells, Antigens, CD4 Antigens, Cell Adhesion, Cell Adhesion Molecules, Humans, Hybridomas, L Cells (Cell Line), Lymphocyte Activation, Mice, T-Lymphocytes, Tetanus Toxin