Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Using an in vitro system we have recently shown that the 3' ends of human pre-snRNAs synthesized by RNA polymerase II are produced by RNA processing directed by the snRNA gene-specific 3' box. Towards a complete characterization of this processing reaction we have further investigated the in vitro requirements for proper 3' end formation of pre-U1 snRNA. Here we show that the 5' cap plays a stimulatory role and processing requires creatine phosphate. Our results also indicate that the pre-U1 processing activity is heat sensitive and that an RNA component is required. In addition, the exact sequence adjacent to the 3' box influences the position of the pre-U1 3' end produced in vitro. Interestingly, the processing extract active for 3'-box-dependent processing also contains an activity that converts the 3' end of RNA containing the U1 Sm protein binding site and the 3' terminal stem-loop into the mature form.

Original publication

DOI

10.1093/nar/gkh619

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

2004

Volume

32

Pages

2987 - 2994

Keywords

Adenosine Triphosphate, Base Sequence, Binding Sites, Coenzymes, Enzyme Stability, HeLa Cells, Hot Temperature, Humans, Molecular Sequence Data, Nucleic Acid Conformation, Phosphocreatine, RNA Caps, RNA Processing, Post-Transcriptional, RNA, Small Nuclear, Ribonucleoproteins