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Human immunodeficiency virus type 1 (HIV-1) can spread between CD4+ T cells by using a virological synapse (VS). The VS assembly is a cytoskeleton-driven process dependent on HIV-1 envelope glycoprotein (Env)-receptor engagement and is hypothesized to require adhesion molecule interactions. Here we demonstrate that leukocyte function-associated antigen 1 (LFA-1), intercellular adhesion molecule 1 (ICAM-1), and ICAM-3 are enriched at the VS and that inhibition of these interactions influences conjugate formation and reduces VS assembly. Moreover, CD4+ T cells deficient in LFA-1 or with modified LFA-1 function were less able to support VS assembly and cell-cell transfer of HIV-1. Thus, cognate adhesion molecule interactions at the VS are important for HIV-1 spread between T cells.

Original publication

DOI

10.1128/JVI.01585-07

Type

Journal article

Journal

J Virol

Publication Date

12/2007

Volume

81

Pages

13916 - 13921

Keywords

Antigens, CD, CD4-Positive T-Lymphocytes, Cell Adhesion Molecules, Flow Cytometry, HIV Infections, HIV-1, Humans, Intercellular Adhesion Molecule-1, Intercellular Junctions, Jurkat Cells, Lymphocyte Function-Associated Antigen-1