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Drosophila plasmatocytes, also known as macrophages, are part of the Drosophila innate immune system and also have roles during development. In late-stage embryos, it is possible to image macrophage migration in situ during development and when they converge at sites of wounding. This protocol describes the isolation of macrophages from third instar Drosophila larvae. The macrophages can be cultured for several hours, and fluorescently labeled macrophages can be screened using a fluorescence-imaging system.

Original publication

DOI

10.1101/pdb.prot5404

Type

Journal article

Journal

Cold Spring Harb Protoc

Publication Date

04/2010

Volume

2010

Keywords

Animals, Cell Separation, Drosophila, Macrophages, Mass Screening, Microscopy, Fluorescence, Staining and Labeling