Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.


Journal article


J Cell Sci

Publication Date





303 - 324


Acridines, Cell Fractionation, Cell Nucleus, Cells, Cultured, Centrifugation, Density Gradient, DNA, Gamma Rays, HeLa Cells, Microscopy, Fluorescence, Nucleic Acid Conformation, Nucleoproteins, Pronase, RNA, Ribonucleases, Sodium Chloride, Species Specificity, Temperature