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Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.

Type

Journal article

Journal

J Cell Sci

Publication Date

11/1976

Volume

22

Pages

303 - 324

Keywords

Acridines, Cell Fractionation, Cell Nucleus, Cells, Cultured, Centrifugation, Density Gradient, DNA, Gamma Rays, HeLa Cells, Microscopy, Fluorescence, Nucleic Acid Conformation, Nucleoproteins, Pronase, RNA, Ribonucleases, Sodium Chloride, Species Specificity, Temperature