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There is an emerging consensus that RNA polymerase II (RNA Pol II) transcription and pre-mRNA processing are tightly coupled events. We show here that exons flanking an intron that has been engineered to be co-transcriptionally cleaved are accurately and efficiently spliced together. These data underline the close coupling of processes in the initial stages of protein-encoding gene expression and provide evidence for a molecular tether connecting emergent splice sites in the pre-mRNA to transcribing RNA Pol II. This observation suggests that for some genes a continuous intron transcript is not required for pre-mRNA splicing in vivo.

Original publication

DOI

10.1016/j.molcel.2006.01.032

Type

Journal article

Journal

Mol Cell

Publication Date

17/03/2006

Volume

21

Pages

849 - 859

Keywords

Animals, Exons, Globins, HeLa Cells, Humans, Introns, Models, Biological, Promoter Regions, Genetic, RNA Polymerase II, RNA Precursors, RNA Splicing, RNA, Catalytic, RNA, Messenger, Transcription, Genetic, Transfection