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We have synthesized four different 5'-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotection and partial purification the 5'-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp) oligoribonucleotides using guanylyl transferase. Radiolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5'-diphosphates to synthetic oligodeoxyribonucleotides and be capable automation.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

25/07/1995

Volume

23

Pages

2641 - 2647

Keywords

Base Sequence, DNA-Directed RNA Polymerases, Influenza A virus, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Oligoribonucleotides, Phosphorylation, RNA, RNA Caps, RNA, Viral