Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Protein kinase A (PKA) is a key regulatory enzyme that, on activation by cAMP, modulates a wide variety of cellular functions. PKA isoforms type I and type II possess different structural features and biochemical characteristics, resulting in nonredundant function. However, how different PKA isoforms expressed in the same cell manage to perform distinct functions on activation by the same soluble intracellular messenger, cAMP, remains to be established. Here, we provide a mechanism for the different function of PKA isoforms subsets in cardiac myocytes and demonstrate that PKA-RI and PKA-RII, by binding to AKAPs (A kinase anchoring proteins), are tethered to different subcellular locales, thus defining distinct intracellular signaling compartments. Within such compartments, PKA-RI and PKA-RII respond to distinct, spatially restricted cAMP signals generated in response to specific G protein-coupled receptor agonists and regulated by unique subsets of the cAMP degrading phosphodiesterases. The selective activation of individual PKA isoforms thus leads to phosphorylation of unique subsets of downstream targets.

Original publication




Journal article


Circ Res

Publication Date





836 - 844


A Kinase Anchor Proteins, Animals, Animals, Newborn, Biosensing Techniques, CHO Cells, Calcium-Binding Proteins, Cricetinae, Cricetulus, Cyclic AMP, Cyclic AMP-Dependent Protein Kinase Type I, Cyclic AMP-Dependent Protein Kinase Type II, Fluorescence Recovery After Photobleaching, Fluorescence Resonance Energy Transfer, GTP-Binding Protein alpha Subunits, Gi-Go, Guanine Nucleotide Exchange Factors, Myocytes, Cardiac, Phosphoric Diester Hydrolases, Phosphorylation, Rats, Rats, Sprague-Dawley, Receptors, G-Protein-Coupled, Recombinant Fusion Proteins, Signal Transduction, Time Factors, Transfection, Troponin I