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Griseofulvin (7-chloro-2',4,6-trimethoxy-6'-methylspiro[benzofuran-2(3H),1'-[2]cyclohexene]-3,4'-dione) induces aggregation of microtubule protein at 0 degrees C. This aggregate contains approx. 90% of the microtubule-associated proteins originally present in the microtubule protein. The supernatant obtained after removal of the griseofulvin-induced aggregate does not form microtubules on warming at 37 degrees C. Addition of the griseofulvin-aggregated protein to this supernatant and warming to 37 degrees C gives rise to a limited amount of microtubule assembly. The possible involvement of griseofulvin-induced aggregation of microtubule protein at 0 degrees C in the inhibition by griseofulvin of microtubule assembly in vitro is discussed.

Type

Journal article

Journal

Biochem J

Publication Date

01/10/1977

Volume

167

Pages

39 - 43

Keywords

Animals, Electrophoresis, Polyacrylamide Gel, Glycoproteins, Griseofulvin, Macromolecular Substances, Microtubules, Nephelometry and Turbidimetry, Sheep, Temperature, Tubulin