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The XMAP215/ch-TOG/Msps family of microtubule-associated proteins (MAPs) promote microtubule growth in vitro and are concentrated at centrosomes in vivo. We show here that Msps (mini-spindles protein) interacts with the centrosomal protein D-TACC, and that this interaction strongly influences microtubule behaviour in Drosophila embryos. If D-TACC levels are reduced, Msps does not concentrate at the centrosomes efficiently and the centrosomal microtubules appear to be destabilized. If D-TACC levels are increased, both D-TACC and Msps accumulate around the centrosomes/spindle poles, and the centrosomal microtubules appear to be stabilized. We show that the interaction between D-TACC and Msps is evolutionarily conserved. We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome.

Original publication




Journal article


Nat Cell Biol

Publication Date





643 - 649


Animals, Blotting, Western, Centrosome, Cloning, Molecular, Drosophila, Drosophila Proteins, Drug Interactions, Evolution, Molecular, Humans, Insect Proteins, Microscopy, Fluorescence, Microtubule-Associated Proteins, Microtubules, Protein Binding, Transfection, Xenopus Proteins