Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Following the demonstration that hybrids between normal B-lymphocytes and myeloma cell lines continue to secrete antibodies with the same specificity as those produced by the parental B-cells, many groups have tried to use this approach to obtain cell lines expressing T-lymphocyte functions by crossing thymoma lines not expressing any measurable activity with various types of T-cell populations. Although there have been reports that hybrids could be isolated which secrete T-cell products with immunological activity, efforts to produce functionally active hybrids from cytolytic T-cells have all been unsuccessful (refs 6, 7, and M. N. and H. D. Engers, unpublished). We have fused an established, T-cell growth factor (TCGF)-dependent murine cytolytic T-lymphocyte (CTL) line with a mouse thymoma line and have obtained hybrids with cytolytic activity when we selected the hybrids in TCGF-containing medium, while hybrids isolated in the absence of growth factor showed no detectable cytolytic potential.

Type

Journal article

Journal

Nature

Publication Date

02/10/1980

Volume

287

Pages

437 - 440

Keywords

Animals, Cell Division, Concanavalin A, Cytotoxicity, Immunologic, Growth Substances, H-2 Antigens, Hybrid Cells, Mice, T-Lymphocytes