Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

WCB is a protein that locates between the inner face of the plasma membrane and the sub-pellicular corset of microtubules in Trypanosoma brucei. We provide the molecular identity of WCB and bioinformatic analysis suggests that it possesses a C2 domain implicated in membrane/protein interactions and a highly charged region possessing characteristics of a putative tubulin-binding domain. Functional analyses via RNA interference (RNAi) depletion show that WCB is essential for cell morphogenesis. Depletion results in gross abnormalities in cell shape, mainly at the cytoskeletal/plasma membrane dynamic posterior end of the trypanosome. Failures in cytokinesis and zoid production are also evident. Furthermore, electron microscopy reveals that RNAi-induced trypanosomes lose local plasma membrane to microtubule corset integrity.

Original publication

DOI

10.1016/j.protis.2007.09.001

Type

Journal article

Journal

Protist

Publication Date

01/2008

Volume

159

Pages

115 - 125

Keywords

Amino Acid Sequence, Animals, Blotting, Western, Cell Membrane, Microscopy, Electron, Transmission, Microtubules, Molecular Sequence Data, Protozoan Proteins, Trypanosoma brucei brucei