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Cdt1 is an essential protein required for licensing of replication origins. Here, we show that in Schizosaccharomyces pombe, Cdt1 is proteolysed in M and G1 phases in response to DNA damage and that this mechanism seems to be conserved from yeast to Metazoa. This degradation does not require Rad3 and Cds1, indicating that it is independent of classic DNA damage and replication checkpoint pathways. Damage-induced degradation of Cdt1 is dependent on Cdt2 and Ddb1, which are components of a Cul4 ubiquitin ligase. We also show that Cdt2 and Ddb1 are needed for cell-cycle changes in Cdt1 levels in the absence of DNA damage. Cdt2 and Ddb1 have been shown to be involved in the degradation of the Spd1 inhibitor of ribonucleotide reductase after DNA damage, and we speculate that Cdt1 downregulation might contribute to genome stability by reducing demand on dNTP pools during DNA repair.

Original publication

DOI

10.1038/sj.embor.7400827

Type

Journal article

Journal

EMBO Rep

Publication Date

11/2006

Volume

7

Pages

1134 - 1139

Keywords

Adaptor Proteins, Signal Transducing, Cell Cycle, Cell Cycle Proteins, Checkpoint Kinase 2, DNA Damage, DNA-Binding Proteins, Hydrolysis, Protein Kinases, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction