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Ultraviolet irradiation of fission yeast cells in G1 phase induced a delay in chromatin binding of replication initiation factors and, consistently, a transient delay in S-phase entry. The cell cycle delay was totally dependent on the Gcn2 kinase, a sensor of the nutritional status, and was accompanied by phosphorylation of the translation initiation factor eIF2alpha and by a general depression of translation. However, the G1-specific synthesis of factors required for DNA replication was not reduced by ultraviolet radiation. The cell cycle delay represents a novel checkpoint with a novel mechanism of action that is not activated by ionizing radiation.

Original publication

DOI

10.1101/gad.421807

Type

Journal article

Journal

Genes Dev

Publication Date

15/03/2007

Volume

21

Pages

649 - 654

Keywords

Cell Cycle Proteins, Enzyme Activation, Eukaryotic Initiation Factor-2, G1 Phase, Minichromosome Maintenance Complex Component 6, Origin Recognition Complex, Phosphorylation, Protein Biosynthesis, Protein-Serine-Threonine Kinases, S Phase, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Ultraviolet Rays