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Selective autophagic degradation of cellular components has been shown to be mediated by the interaction of LIR motif-containing proteins with ATG8-family proteins. Here, we present a detailed methodology for the in silico evaluation of potential binding between LIR motif-containing proteins and ATG8-family proteins. We visualize AlphaFold-predicted protein complexes using PyMOL to assess potential interactions, providing an effective computational tool for this purpose.

Original publication

DOI

10.1007/978-1-0716-4067-8_16

Type

Chapter

Publication Date

2024

Volume

2845

Pages

197 - 201

Keywords

AlphaFold, Atg8-family proteins, LIR motif, p62, Autophagy-Related Protein 8 Family, Protein Binding, Amino Acid Motifs, Computer Simulation, Computational Biology, Autophagy, Humans, Software, Protein Interaction Domains and Motifs