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Here we describe methods for the characterization of the kinesin-9 family motor protein Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis. Here we outline biochemical assays for studying the interaction of Rabkinesin-6/Rab6-KIFL/MKlp2 with microtubules, and its regulation and interaction with the mitotic polo-like kinase 1 using recombinant proteins expressed in and purified from insect cells. Protocols for the in vivo functional analysis of Rabkinesin-6/Rab6-KIFL/MKlp2 using depletion with small interfering RNA duplexes are described.

Original publication

DOI

10.1016/S0076-6879(05)03054-5

Type

Journal article

Journal

Methods Enzymol

Publication Date

2005

Volume

403

Pages

618 - 628

Keywords

Animals, Blotting, Western, Cell Cycle, Humans, Kinesin, Ligands, Microtubules, Phosphorylation, RNA Interference, Recombinant Proteins, Spodoptera