Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The budding yeast spindle pole body (SPB) not only organizes the astral and nuclear microtubules but is also associated with a number of cell-cycle regulators that control mitotic exit. Here, we describe that the core SPB component Nud1p is a key protein that functions in both processes. The astral microtubule organizing function of Nud1p is mediated by its interaction with the gamma-tubulin complex binding protein Spc72p. This function of Nud1p is distinct from its role in cell-cycle control: Nud1p binds the spindle checkpoint control proteins Bfa1p and Bub2p to the SPB, and is part of the mitotic exit network (MEN) in which it functions upstream of CDC15 but downstream of LTE1. In conditional lethal nud1-2 cells, the MEN component Tem1p, a GTPase, is mislocalized, whereas the kinase Cdc15p is still associated with the SPB. Thus, in nud1-2 cells the failure of Tem1p to interact with Cdc15p at the SPB probably prevents mitotic exit.

Original publication

DOI

10.1093/emboj/19.23.6475

Type

Journal article

Journal

EMBO J

Publication Date

01/12/2000

Volume

19

Pages

6475 - 6488

Keywords

Animals, Antineoplastic Agents, Cell Cycle, Cell Cycle Proteins, Cell Line, Cell Nucleus, Cytoskeletal Proteins, Deoxyribonucleases, Flow Cytometry, Fungal Proteins, GTP-Binding Proteins, Green Fluorescent Proteins, Guanine Nucleotide Exchange Factors, Insecta, Luminescent Proteins, Microscopy, Electron, Microscopy, Fluorescence, Microtubules, Mitosis, Monomeric GTP-Binding Proteins, Mutation, Nocodazole, Phenotype, Phosphorylation, Protein Binding, Saccharomyces cerevisiae Proteins, Suppression, Genetic, Temperature, Time Factors, Tubulin, Two-Hybrid System Techniques, tRNA Methyltransferases