Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have examined the extent of allelic exclusion at the T cell receptor (TCR) beta locus using monoclonal antibodies specific for V beta products. A small proportion (approximately 1%) of human peripheral blood T cells express two V beta as determined by flow cytometric analysis, isolation of representative clones, and sequencing of the corresponding V beta chains. Dual beta T cells are present in both the CD45R0+ and CD45R0- subset. These results indicate that dual beta expression is compatible with both central and peripheral selection. They also suggest that the substantial degree of TCR beta allelic exclusion is dependent only on asynchronous rearrangements at the beta locus, whereas the role of the pre-TCR is limited to signaling the presence of at least one functional beta protein.

Type

Journal article

Journal

J Exp Med

Publication Date

01/04/1995

Volume

181

Pages

1587 - 1591

Keywords

Alleles, Amino Acid Sequence, Antibodies, Monoclonal, Antigens, CD45, Base Sequence, Cell Separation, Diploidy, Flow Cytometry, Gene Expression Regulation, Humans, Immunophenotyping, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Antigen, T-Cell, alpha-beta, Signal Transduction, T-Lymphocyte Subsets