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The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome.

Original publication

DOI

10.1126/science.1206097

Type

Journal article

Journal

Science

Publication Date

23/09/2011

Volume

333

Pages

1758 - 1760

Keywords

Adenosine Triphosphatases, Adenosine Triphosphate, Chromatin Assembly and Disassembly, DNA, Fungal, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Genome, Fungal, Mutation, Nucleosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Initiation Site