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A 1000-fold purification of human T-cell growth factor (TCGF) was achieved starting from supernatants of human spleen cells stimulated with phytohaemagglutinin (PHA) in culture medium containing 0.5% serum. The purification scheme involved precipitation with ammonium sulphate, gel filtration and blue-Sepharose chromatography. The use of polyethylene glycol 6000 (PEG 6000) was critical during the chromatographic steps in order to obtain high final recoveries or activity (40-50%). Purified preparations of TCGF labelled with 125I by the chloramine T method revealed that the activity co-migrated with 2 molecular species of 14,000-17,000 daltons in SDS-PAGE under non-reducing conditions.

Type

Journal article

Journal

J Immunol Methods

Publication Date

27/08/1982

Volume

53

Pages

15 - 26

Keywords

Animals, Cell Line, Chemical Precipitation, Chromatography, Agarose, Chromatography, Gel, Culture Media, Dialysis, Electrophoresis, Polyacrylamide Gel, Humans, Interleukin-2, Mice, Spleen, T-Lymphocytes, Cytotoxic