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Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin's stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclusively monomeric. The monomeric stoichiometry of sororin:cohesin complexes demonstrates that sister chromatid cohesion is conferred by individual cohesin rings, a key prediction of the proposal that cohesion arises from the co-entrapment of sister DNAs.

Original publication

DOI

10.1126/science.adl4606

Type

Journal article

Journal

Science

Publication Date

08/03/2024

Volume

383

Pages

1122 - 1130

Keywords

Humans, Cell Cycle Proteins, Chromatids, Chromatin, Cohesins, DNA, Sister Chromatid Exchange, Cell Line, Tumor