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We have determined the extent of the primary transcription unit for the two highly expressed genes nmt1 and nmt2 of Schizosaccharomyces pombe. Transcription run-on analysis in permeabilized yeast cells was employed to map polymerase density across the 3'-flanking region of these two genes. Surprisingly, polymerases were detected 4.3 kb beyond the nmt1 polyadenylation [poly(A)] site and 2.4 kb beyond the nmt2 poly(A) site, which in each case have transcribed through an entire convergent downstream transcription unit. However, the steady-state levels of both downstream genes were unaffected by the high level of nmt1 or nmt2 nascent transcription. Analysis of nmt1 and nmt2 RNA 3' end formation signals indicates that efficient termination of transcription requires not only a poly(A) signal but also additional pause elements. The absence of such pause elements close to the poly(A) sites of these genes may account for their extended nascent transcripts.

Original publication




Journal article



Publication Date





3066 - 3077


Base Sequence, Chromosome Mapping, Fungal Proteins, Genes, Fungal, Genes, Overlapping, Molecular Sequence Data, Peptide Chain Termination, Translational, Poly A, RNA, Fungal, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Thiamine, Transcription, Genetic