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Monoclonal antibody (McAb) H9/25 has previously been shown to react with an alloantigen expressed on subpopulations of mouse lymphocytes. We here investigated the expression of the antigen (H9/25 Ag) on functional subsets of T and B cells. Lymphocytes were depleted of H9/25 Ag-bearing cells by complement-dependent cytolysis or by the affinity to immobilized McAb H9/25, and the residual immunological functions were tested. In these tests, killer T cells generated in mixed lymphocyte cultures and their precursors were found to express H9/25 Ag. In contrast, the activity and frequency of helper T cells specific to keyhole limpet hemocyanin carrier were not decreased by the depletion of H9/25 Ag-bearing cells. On the other hand, IgG and IgM anti-TNP plaque-forming cells were found to express H9/25 Ag, while it was not detected on unprimed B cells, as tested by using TNP-Ficoll and TNP-lipopolysaccharide. Only a small proportion of TNP memory B cells seemed to express the antigen. These results revealed significant differences between H9/25 Ag and other known alloantigens in the distribution among functional subsets of lymphocytes.

Original publication

DOI

10.1002/eji.1830100704

Type

Journal article

Journal

Eur J Immunol

Publication Date

07/1980

Volume

10

Pages

503 - 509

Keywords

Animals, Antibodies, Antibody Formation, Antibody-Producing Cells, B-Lymphocytes, Clone Cells, Concanavalin A, Immunologic Memory, Killer Cells, Natural, Lymphocyte Culture Test, Mixed, Mice, Mice, Inbred C3H, Mice, Inbred CBA, T-Lymphocytes