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An antiglobulin-coupled red cell assay is described for screening monoclonal antibodies against cell surface antigens. A monoclonal antibody specific for rat immunoglobulin kappa chains was coupled to red blood cells and used to detect binding of rat monoclonal antibodies to cells attached to the wells of microtitre plates. The method was found to be simpler and more rapid than the alternative enzyme-linked binding assay and useful for rapid screening and selection of antibodies for use as differentiation markers of human and mouse haemopoietic cells.

Type

Journal article

Journal

J Immunol Methods

Publication Date

20/01/1984

Volume

66

Pages

81 - 87

Keywords

Animals, Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Antigens, Surface, Binding Sites, Antibody, Blood Group Antigens, Bone Marrow Cells, Enzyme-Linked Immunosorbent Assay, Hemadsorption, Humans, Immunologic Techniques, Leukemia, Myeloid, Acute, Mice, Rats, Sheep, T-Lymphocytes