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The structural analysis of the 2.0 kb region upstream from the epsilon-globin gene has been carried out. A genomic DNA map around the gene was worked out in some detail to ensure that the cloned DNA was representative of the actual chromosomal arrangement. Furthermore, a new technique was developed to precisely map a reiterated DNA sequence present 1.5 kb to the 5' side of the gene. The complete nucleotide sequence of the 2.0 kb 5' flanking region was then determined and overlapped with the gene. The sequence included the reiterated DNA sequence which is homologous to the so-called AluI family of repeats. Unusual stretches of sequence 50 nucleotides long, where A + T represent about 90% of the bases, are present at both the 5' and 3' sides of the repeat.


Journal article


Nucleic Acids Res

Publication Date





4393 - 4404


Base Sequence, Chromosomes, Human, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant, Genes, Globins, Humans, Molecular Weight, Nucleic Acid Hybridization