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The direct association between messenger RNA (mRNA) 3'-end processing and the termination of transcription was established for the CYC1 gene of Saccharomyces cerevisiae. The mutation of factors involved in the initial cleavage of the primary transcript at the poly(A) site (RNA14, RNA15, and PCF11) disrupted transcription termination at the 3' end of the CYC1 gene. In contrast, the mutation of factors involved in the subsequent polyadenylation step (PAP1, FIP1, and YTH1) had little effect. Thus, cleavage factors link transcription termination of RNA polymerase II with pre-mRNA 3'-end processing.

Type

Journal article

Journal

Science

Publication Date

10/04/1998

Volume

280

Pages

298 - 301

Keywords

Cytochrome c Group, Cytochromes c, Fungal Proteins, Genes, Fungal, Mutation, Nuclear Proteins, Poly A, RNA Polymerase II, RNA Precursors, RNA, Fungal, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Temperature, Transcription, Genetic, mRNA Cleavage and Polyadenylation Factors