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In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.

Original publication

DOI

10.1007/978-1-0716-2477-7_19

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2022

Volume

2528

Pages

289 - 303

Keywords

DNA Damage, Double Strand breaks, Proximity Ligation Assay, R-loops, RNA modification, RNA:DNA hybrids